| Autor: |
Arya, Inder Dev1, Satsangi, Richa2, Arya, Sarita3 |
| Předmět: |
|
| Zdroj: |
Journal of Sustainable Forestry. 2002, Vol. 14 Issue 2/3, p103. 12p. |
| Abstrakt: |
Micropropagation protocols for Dendrocalamus asper using nodal shoots and seeds culture are described. Multiple shoots were induced through forced axillary branching. Ninety-five percent of the nodal shoot explants taken from juvenile primary and lateral branches, produced multiple shoots through axillary buds activation within 2 to 8 weeks on Murashige & Skoog's (MS) medium supplemented with 0.1-15 mg/l benzyladenine (BA). The cultured seeds also produced multiple shoots (1-20) within 6 weeks on this medium. The multiple shoot differentiation was influenced by the concentration of BA in the medium. The in vitro generated shoots were excised and subculture on MS + 3.0 mg/l BAP for further shoot multiplication. Fifteen to 20 fold rate of shoot multiplication was achieved by regular subculturing. These shoots were multiplied for more than 3 years without loss of vigor. Ninety-five percent of the shoots were rooted, when propagules (each consisting of cluster of 3 shoots) were transferred on to MS medium with 3.0 mg/l NAA or 10 mg/l indole-3-butyric acid (IBA). To date, 18,000 plants (through axillary bud initiated from nodal explants) and 6,000 plants from seed culture have been hardened and acclimatized. 12,000 plants have been field transferred. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
|
