| Autor: |
Khan, Mohd Jahir1 (AUTHOR) khanmohdjahir@gmail.com, Ahmad, Abrar2 (AUTHOR), Zamzami, Mazin A.2 (AUTHOR), Siddiqui, Sahabjada3 (AUTHOR), Khan, Mahmood Ahmad4 (AUTHOR) |
| Předmět: |
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| Zdroj: |
Clean Technologies & Environmental Policy. Nov2024, Vol. 26 Issue 11, p3955-3964. 10p. |
| Abstrakt: |
The broad antibacterial activity range of silver nanoparticles (AgNPs) has drawn considerable interest. However, due to a lack of knowledge about toxicity, its potential uses are limited. Therefore, the current study attempted to increase the antibacterial activity of AgNPs without any detrimental effect on cells. Monodispersed, spherical AgNPs with an average particle size of 35 nm were chemically synthesized and capped with β-cyclodextrin. Native and β-cyclodextrin-capped silver nanoparticles (βCD-AgNPs) were characterized by UV–vis spectroscopy and transmission electron microscopy. Capping of βCD on AgNPs was examined by 1H NMR spectroscopy. The toxicity of native and βCD-AgNPs was evaluated on fibroblasts and glioblastoma cells. Native AgNPs showed the highest reduction in ATP content as compared to the capped βCD-AgNPs in the cell viability assay. DNA damage was measured by comet assay using human lymphocytes, which demonstrated that the toxicity of native AgNPs was higher than the βCD-AgNPs. The antimicrobial activity of native and βCD-AgNPs was studied with gram-negative bacteria (Escherichia coli) and gram-positive bacteria (Bacillus subtilis), which showed that the antimicrobial activity of βCD-AgNPs was remarkably higher than the native AgNPs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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Full text from SpringerLink