| Abstrakt: |
The present work focused on the isolation of a bacterial strain multi-resistant to heavy metals with a high potential for reducing hexavalent chromium (Cr(VI)) and studied its Cr(VI) removal performance in immobilized state and the mechanisms involved. Bacterial isolate was identified as Bacillus safensis CCMM B629 (B. safensis), is able to completely reduce 50, 100 and 200 mg/L of Cr(VI) after 24, 48 and 120 h, respectively under optimized conditions of pH 7 and 30°C. The coexistence of nitrates, cadmium and mercury inhibits reduction, while copper and iron significantly improve removal efficiencies. Additionally, the presence of electron donors such as glycerol, glucose and citrate significantly increases bioreduction rate. Cells immobilized in alginate beads successfully reduced Cr(VI) compared to free cells, showing the performance of biobeads in Cr(VI) reduction. Membrane fraction exhibited highest rate of Cr(VI) reduction (65%) compared to other cellular components, indicating that Cr(VI) reduction occurred primarily in cell membrane. Further characterization of Cr(VI) removal by B. safensis cells using scanning electron microscopy and energy-dispersive X-ray (SEM-EDX) analysis showed its ability to reduce and adsorb Cr(VI), confirming that hexavalent chromium was taken up successfully on bacterial cell surfaces. Based on Fourier transform infrared spectroscopy analysis (FTIR), hydroxyl, carboxyl, amide, and phosphoryl functional groups participated in combination with Cr(III). In conclusion, B. safensis is a bacterium with great potential for Cr(VI) removal, and it is a promising and competitive strain for use in bioremediation of Cr(VI) contaminated industrial effluents. [ABSTRACT FROM AUTHOR] |
