| Autor: |
Shah, Parth N.1 (AUTHOR), Niermans, Kelly1,2 (AUTHOR) kelly.niermans@wur.nl, Hoek- van den Hil, Elise F.2 (AUTHOR), Dicke, Marcel1 (AUTHOR), van Loon, Joop J.A.1 (AUTHOR) |
| Předmět: |
|
| Zdroj: |
Pesticide Biochemistry & Physiology. Jun2024, Vol. 202, pN.PAG-N.PAG. 1p. |
| Abstrakt: |
Contamination of food products with mycotoxins such as aflatoxin B 1 (AFB 1) poses a severe risk to human health. Larvae of the black soldier fly (BSFL), Hermetia illucens (Diptera: Stratiomyidae), can successfully metabolize AFB 1 without any negative consequences on their survival or growth. Organic waste streams contaminated with mycotoxins can be upcycled into protein-rich BSFL as an alternative feed for livestock and the left-over feed residue into nutrient-rich crop fertilizers. However, the underlying mechanisms that allow BSFL to metabolize AFB 1 are unknown. In this study, five-day-old BSFL were fed with either a control or an AFB 1 -spiked (20 μg/kg) diet to elucidate the underlying mechanisms. Larval samples were collected at three timepoints (6 h, 24 h and 72 h) and subjected to RNA-Seq analysis to determine gene expression patterns. Provision of an AFB 1 -spiked diet resulted in an up-regulation of 357 and a down-regulation of 929 unique genes. Upregulated genes include multiple genes involved in AFB 1 metabolism in other (insect) species. Downregulated genes were generally involved in the insects' growth, development, and immunity. BSFL possesses a diverse genetic arsenal that encodes for enzymes capable of metabolizing AFB 1 without trade-offs on larval survival. In conclusion, the adverse impact of AFB 1 exposure on immunity-related processes is observed in the transcriptomic response, and is indicative of a trade-off between detoxification and immune responses. [Display omitted] • BSFL harbour a diverse genetic arsenal encoding enzymes for AFB 1 metabolism. • AFB 1 feeding results in up- and down-regulation of 357 and 929 unique genes. • Genes known to be involved in AFB 1 metabolism were upregulated. • Genes involved in the insects' growth, development, and immunity were downregulated. • AFB 1 feeding causes trade-off in metabolic and immunity related gene expression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
|
Full Text from ScienceDirect