| Autor: |
Annunziato, Mark1,2,3 (AUTHOR), Bashirova, Narmin4 (AUTHOR), Eeza, Muhamed N.H.5 (AUTHOR), Lawson, Ariel2 (AUTHOR), Fernandez‐Lima, Francisco1,2,3 (AUTHOR), Tose, Lilian V.1,2,3 (AUTHOR), Matysik, Jörg4 (AUTHOR), Alia, A.5,6 (AUTHOR), Berry, John P.1,2,3 (AUTHOR) berryj@fiu.edu |
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| Zdroj: |
Environmental Toxicology & Chemistry. Apr2024, Vol. 43 Issue 4, p896-914. 19p. |
| Abstrakt: |
Known for their high stability and surfactant properties, per‐ and polyfluoroalkyl substances (PFAS) have been widely used in a range of manufactured products. Despite being largely phased out due to concerns regarding their persistence, bioaccumulation, and toxicity, legacy PFAS such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid continue to persist at high levels in the environment, posing risks to aquatic organisms. We used high‐resolution magic angle spinning nuclear magnetic resonance spectroscopy in intact zebrafish (Danio rerio) embryos to investigate the metabolic pathways altered by PFOS both before and after hatching (i.e., 24 and 72 h post fertilization [hpf], respectively). Assessment of embryotoxicity found embryo lethality in the parts‐per‐million range with no significant difference in mortality between the 24‐ and 72‐hpf exposure groups. Metabolic profiling revealed mostly consistent changes between the two exposure groups, with altered metabolites generally associated with oxidative stress, lipid metabolism, energy production, and mitochondrial function, as well as specific targeting of the liver and central nervous system as key systems. These metabolic changes were further supported by analyses of tissue‐specific production of reactive oxygen species, as well as nontargeted mass spectrometric lipid profiling. Our findings suggest that PFOS‐induced metabolic changes in zebrafish embryos may be mediated through previously described interactions with regulatory and transcription factors leading to disruption of mitochondrial function and energy metabolism. The present study proposes a systems‐level model of PFOS toxicity in early life stages of zebrafish, and also identifies potential biomarkers of effect and exposure for improved environmental biomonitoring. Environ Toxicol Chem 2024;43:896–914. © 2024 SETAC [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
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