Evaluating the Effects of Chronic Oral Exposure to the Food Additive Silicon Dioxide on Oral Tolerance Induction and Food Sensitivities in Mice.

Autor: Lamas, Bruno1 bruno.lamas@inrae.fr, Breyner, Natalia Martins1, Malaisé, Yann1, Wulczynski, Mark2, Galipeau, Heather J.2, Gaultier, Eric1, Cartier, Christel1, Verdu, Elena F.2, Houdeau, Eric1 eric.houdeau@inrae.fr
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Zdroj: Environmental Health Perspectives. Feb2024, Vol. 132 Issue 2, p027007-1-027007-14. 14p.
Abstrakt: BACKGROUND: The increasing prevalence of food sensitivities has been attributed to changes in gut microenvironment; however, ubiquitous environmental triggers such as inorganic nanoparticles (NPs) used as food additives have not been thoroughly investigated. OBJECTIVES: We explored the impact of the NP-structured food-grade silicon dioxide (푓푔-SiO2) on intestinal immune response involved in oral tolerance (OT) induction and evaluated the consequences of oral chronic exposure to this food-additive using a mouse model of OT to ovalbumin (OVA) and on gluten immunopathology in mice expressing the celiac disease risk gene, HLA-DQ8. METHODS: Viability, proliferation, and cytokine production of mesenteric lymph node (MLN) cells were evaluated after exposure to 푓푔-SiO2. C57BL/ 6J mice and a mouse model of OT to OVA were orally exposed to 푓푔-SiO2 or vehicle for 60 d. Fecal lipocalin-2 (Lcn-2), anti-OVA IgG, cytokine production, and immune cell populations were analyzed. Nonobese diabetic (NOD) mice expressing HLA-DQ8 (NOD/DQ8), exposed to 푓푔-SiO2 or vehicle, were immunized with gluten and immunopathology was investigated. RESULTS: MLN cells exposed to 푓푔-SiO2 presented less proliferative T cells and lower secretion of interleukin 10 (IL-10) and transforming growth factor beta (TGF-β) by T regulatory and CD45+ CD11b+ CD103+ cells compared to control, two factors mediating OT. Mice given  푓𝑔-SiO2 exhibited intestinal Lcn-2 level and interferon gamma (IFN-γ) secretion, showing inflammation and less production of IL-10 and TGF-β. These effects were also observed in OVA-tolerized mice exposed to 푓푔-SiO2, in addition to a breakdown of OT and a lower intestinal frequency of T cells. In NOD/DQ8 mice immunized with gluten, the villus-to-crypt ratio was decreased while the CD3+ intraepithelial lymphocyte counts and the Th1 inflammatory response were aggravated after 푓푔-SiO2 treatment. DISCUSSION: Our results suggest that chronic oral exposure to 푓푔-SiO2 blocked oral tolerance induction to OVA, and worsened gluten-induced immunopathology in NOD/DQ8 mice. The results should prompt investigation on the link between SiO2 exposure and food sensitivities in humans. [ABSTRACT FROM AUTHOR]
Databáze: GreenFILE
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