| Abstrakt: |
During the normal production of ethanol from pretreated lignocellulosic biomass, only the solid fraction containing C6 sugars is generally used by unmodified yeasts in the fermentation process – the remaining liquid fraction, rich in xylose, is used almost exclusively by genetically modified organisms (GMOs) or for other purposes. As the liquor can be used in the manufacture of a series of chemical inputs, microorganisms that metabolize C5 sugars from different sources were initially isolated with the aim of converting these sugars into acetic acid (AA) and lactic acid (LA). Experiments were carried out with pretreated elephant grass liquor, evaluating the effect of adding commonly referenced nutrients through experimental design. Thus, the present work involves the isolation, screening, and molecular characterization of bacteria, easily found in nature, with the potential to metabolize xylose and produce AA and LA, with yields similar or superior to those shown in studies employing GMOs. Using wild‐type strains, high yields were obtained with Acetobacter cerevisiae (0.96 g LA and 0.71 g AA per g total sugars) and Levilactobacillus brevis (0.77 g LA and 0.61 g AA per g total sugars) in relation to the organic acids of interest, highlighting the total conversion of the sugar blend (xylose, glucose, and arabinose) in 24 h. Ammonium citrate also significantly influenced the final yields, being responsible for the increase in production. The results are useful for biorefinery platforms and may contribute to the full use of sugars released in the lignocellulosic pretreatment of raw materials. [ABSTRACT FROM AUTHOR] |
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