ANTIBACTERIAL EFFECTIVENESS AND POTENTIAL APPLICATION OF LDPE FILM CONTAINING Leuconostoc BACTERIOCINS TO CONTROL SPOILAGE AND PATHOGENIC BACTERIA IN RAW BEEF MEAT.

Autor: Chahrour, Wassila1,2 chahrourw@hotmail.fr, Bellil, Yahia1,2, Benabbou, Taha Ahmed1, Benreguieg, Mokhtar1, Benmechernene, Zineb2, Kihal, Mebrouk2
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Zdroj: Fresenius Environmental Bulletin. Oct2023, Vol. 32 Issue 10, p3103-3115. 13p.
Abstrakt: The preservation of raw meat products is a paramount concern within the food industry, spoilage and pathogenic bacteria pose significant challenges in maintaining the freshness and safety of raw beef meat. This study was focused on the exploration of the effectiveness of partially purified bacteriocins (ppBA) from three Leuconostoc strains isolated from camel's milk for raw beef meat preservation by active packaging film. The active packaging film containing ppBA bacteriocin was developed using lowdensity polyethylene LDPE film as the packing material through a soaking method. After a proteomic characterization the identification of distinct Leuconostoc strains as Leuconostoc lactis TW88, Leuconostoc mesenteroides KW83, and Leuconostoc mesenteroides BW46. All the tested strains were active against more than four indicators' bacteria presented by an inhibition zone up to 20mm with Bacillus cereus, 23,5mm with Enterococcus faecalis, 22,5mm with Listeria innocua, 25mm with Pseudomonas aeruginosa and 23,5mm with Escherichia coli. The activated film demonstrated antibacterial effects against seven pathogenic bacteria. When in contact with the meat matrix, the active LDPE film released the bacteriocins, which exhibited residual activity under various simulated conditions. These activated films displayed biopreservative efficacy in controlling the growth of pathogens in meat. The bacteriocin-activated film can be utilized as a packing material to control the growth of spoilage and pathogenic bacteria in food, ultimately extending the shelf life of food products. [ABSTRACT FROM AUTHOR]
Databáze: GreenFILE