A Standardized Protocol for Measuring Bioelectrical Impedance in Green Turtles (Chelonia mydas).

Autor: Kophamel, Sara1 (AUTHOR) sara.kophamel@my.jcu.edu.au, Ward, Leigh C.2 (AUTHOR), Ariel, Ellen1 (AUTHOR), Mendez, Diana3 (AUTHOR), O'Brien, Lauren M.4 (AUTHOR), Burchell, Lauren1 (AUTHOR), Munns, Suzanne L.1 (AUTHOR)
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Zdroj: Physiological & Biochemical Zoology. Mar/Apr2023, Vol. 96 Issue 2, p87-99. 13p. 1 Color Photograph, 1 Diagram, 5 Charts, 1 Graph.
Abstrakt: Bioelectrical impedance analysis (BIA) is gaining popularity in wildlife studies as a portable technology for immediate and nondestructive predictions of body composition components, such as fat-free and fat masses. Successful application of BIA for field-based research requires the identification and control of potential sources of error, as well as the creation of and adherence to a standardized protocol for measurement. The aim of our study was to determine sources of error and to provide a standardization protocol to improve measurement precision of BIA on juvenile green turtles (Chelonia mydas ; n = 35). We assessed the effects of altered environmental temperature (20°C–30°C), postprandial state (2–72 h), and time out of the water (2 h) on five impedance parameters (resistance at infinite frequency [ R inf], resistance at zero frequency [ R 0], resistance at 50 kHz [ R 50], phase angle at 50 kHz [PhA50], and intracellular resistance [ R i]) using a bioimpedance spectroscopy device. Technical reproducibility of measurements and interanimal variability were also assessed. We found an inverse exponential relationship between change in environmental temperature and impedance parameters R inf, R 0, and R 50. Postprandial state significantly increased R inf and R i 72 h after feeding. BIA measurements were reproducible within individual juvenile green turtles at temperatures from 20°C to 30°C. Significant variation in impedance values was found between animals at all temperatures, sampling times, and postprandial states, but the relative differences (%) were small in magnitude. Our study suggests that measurement precision is improved by measuring animals at consistent environmental temperatures close to their preferred thermal range. We propose a standardized protocol of measurement conditions to facilitate laboratory and field use of BIA for body composition assessment studies in turtles. [ABSTRACT FROM AUTHOR]
Databáze: GreenFILE
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