| Autor: |
Kajihara, Kimberly K.1, Amaral, John A.1 amaral@usfca.edu, Toia, Robert F.2 |
| Předmět: |
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| Zdroj: |
Environmental Toxicology & Chemistry. Sep2000, Vol. 19 Issue 9, p2235-2238. 4p. 1 Chart, 2 Graphs. |
| Abstrakt: |
Methane consumption by methanotrophic bacteria was previously shown to be temporarily inhibited by α-pinene. Based on literature considerations, loss of inhibition may be due to bacterial degradation of the monoterpene to α-pinene oxide, an anticipated metabolite. However, since α-pinene oxide is unstable in aqueous media, detection of its production by methanotrophs or other bacteria is problematic. Therefore, we used gas chromatography-mass spectrometry analysis to study the chemical breakdown of α-pinene oxide in various buffer systems (Tris[hydroxymethyl]aminomethane, 3-[N-morpholino]propanesulfonic acid, phosphate; pH 7--9) suitable for bacterial whole-cell and cell-free experiments. In every case, aqueous phase α-pinene oxide was unstable and its disappearance was accompanied by the appearance of five decomposition products in a characteristic fingerprint that was in part buffer dependent. However, this fingerprint was adequately stable in phosphate buffer such that its appearance could be used to infer the intermediacy of α-pinene oxide if produced by the bacteria at or near their optimal pH. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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