| Autor: |
Katsube, Takanori1 (AUTHOR) katsube.takanori@qst.go.jp, Wang, Bing1 (AUTHOR), Tanaka, Kaoru1 (AUTHOR), Ninomiya, Yasuharu1 (AUTHOR), Hirakawa, Hirokazu2 (AUTHOR), Liu, Cuihua2 (AUTHOR), Maruyama, Kouichi1 (AUTHOR), Vares, Guillaume3 (AUTHOR), Liu, Qiang4 (AUTHOR), Murakami, Masahiro1 (AUTHOR), Nakajima, Tetsuo1 (AUTHOR), Fujimori, Akira2 (AUTHOR), Nenoi, Mitsuru1,5 (AUTHOR) nenoi.mitsuru@qst.go.jp |
| Předmět: |
|
| Zdroj: |
Mutation Research - Genetic Toxicology & Environmental Mutagenesis. Oct2022, Vol. 882, pN.PAG-N.PAG. 1p. |
| Abstrakt: |
High atomic number and energy (HZE) particles such as iron-56 (Fe) ions are a major contributor to health risks in long-term manned space exploration. The aim of this study is to understand radiation-induced differential genotoxic effects between HZE particles and low linear energy transfer (LET) photons. C57BL/6J Jms female mice of 8 weeks old were exposed to total body irradiation of accelerated Fe-particles with a dose ranging from 0.1 to 3.0 Gy or of X-rays with a dose ranging from 0.1 to 5.0 Gy. Chromosomal aberrations (CAs) in splenocytes were examined by fluorescence in situ hybridization at 1- and 2-months after exposure. Clonal expansions of cells with CAs were found to be induced only by X-rays but not by Fe-particles. Dose-dependent increase in the frequencies of stable-type CAs was observed at 1- as well as 2-months after exposure to both radiation types. The frequencies of stable-type CAs in average were much higher in mice exposed to X-rays than those to Fe-particles and did not change significantly between 1- and 2-months after exposure to both radiation types. On the other hand, the frequencies of unstable-type CAs induced by X-rays and Fe-particles were not much different, and they appeared to decrease with time from 1- to 2-months after exposure. These results suggested that larger fraction of stable-type CAs induced by Fe-particles might be non-transmissible than those by X-rays because of some associating lethal alterations on themselves or on other chromosomes in the same cells and that these cells might be removed by 1-month after Fe-TBI. We also demonstrated that exposure to Fe-particles induced insertions at relatively higher frequency to other stable-type CAs than X-rays. Our findings suggest that insertions can be used as indicators of past exposure to high-LET particle radiation. • Long term cytogenic effects of Fe ion particles were analyzed in vivo. • Clonal expansions of aberrant splenocytes are induced by X-rays but not by Fe ions. • Chromosome aberrations induced by Fe ions don't persist so long as those by X-rays. • Fe ions induce insertions at relatively higher frequency than X-rays. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
|
Full Text from ScienceDirect