| Autor: |
Xiong, Jingyuan1 (AUTHOR), Wang, Minghui1 (AUTHOR), Tian, Ye1 (AUTHOR), Ling, Aru2 (AUTHOR), Shan, Shufang2 (AUTHOR), Zhao, Li1 (AUTHOR), Cheng, Guo2 (AUTHOR) |
| Předmět: |
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| Zdroj: |
Toxicological & Environmental Chemistry. Jan2022, Vol. 104 Issue 1, p129-140. 12p. |
| Abstrakt: |
S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (±)-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the effect and mechanism of (±)-equol on the secretion of gonadotropin-releasing hormone. The cells were treated with up to 20 µmol/L of (±)-equol to determine the influence on cell viability. Enzyme-linked immunosorbent assay, real-time quantitative polymerase chain reaction, and Western-blotting analysis were used to investigate the impacts of (±)-equol on the secretion and transcription of gonadotropin-releasing hormone, and the transcription and translation of key neuromodulators. This study showed that (±)-equol could decrease cell viability, increase the ability to secrete gonadotropin-releasing hormone, and interfere with the expression of neurokinin B receptor, makorin ring finger protein 3, and silent information regulator 1. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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