| Autor: |
Ai, Abdel-Hamid A.1, Galal, Ola A.1, Ebrahim, Hamdi B. A.1, Zedan, Amina M. G.2, Aboulila, Aziza A.1 aziza.aboulila@agr.kfs.edu.eg |
| Předmět: |
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| Zdroj: |
Fresenius Environmental Bulletin. Apr2022, Vol. 31 Issue 4, p4273-4286. 14p. |
| Abstrakt: |
Compounds presented in propolis may target proteins engaged in regulation of gene expression. Sixty males of albino rats classified into five groups were used to evaluate nephron-protective effect of propolis against gentamicin toxicity. Gene expression was used as a tool to identify the role of propolis in reducing gentamicin-side effects. The study was designed as G 1: animals received only saline as a control, G2: animals given propolis orally by stomach tube (500 mg/kg b.w) for eight days, G3: animals received daily intraperitoneal injection of gentamicin (100 mg/kg b.w) for eight days, G4: animals treated with propolis after one hour from injection by gentamicin and G5: animals treated with propolis after finishing injection by gentamicin and continued to the end of the experiment. Half of all animals were sacrificed after three weeks (1st period) and the others after six weeks (2nd period). Results indicated that propolis effect after one week from treatment by gentamicin was better than one hour in both of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Creatinine, urea levels and malondialdehyde (NIDA) activity were significantly decreased with using propolis supplementation in gentamicin-treated rats. Propolis increased significantly the level of total antioxidant capacity (TAC) in both 1 h (G4) and 1 weak (G5) but the increase in GS was more than G4 in the two periods. Concerning gene expression analysis of inflammation-related genes (tumour necrosis factor a; TNF a and nuclear factor-kappa B; NFKB), kidney regeneration-related gene (Nephrin) and apoptosis-related gene (Caspase 3), propolis treatments showed increased expression of TNF a and NFKB which significantly down-regulated following administration of propolis for 1 h (G4) and 1 weak (G5). Treatment with propolis stimulated Nephrin gene and enhanced the expression in the first and second periods, whereas reduced the increasing expression of Caspase 3 that induced by gentamicin in the first and second periods. Practically use of propolis inhibits histopathological toxic effect of gentamicin on kidney tissue. In conclusions, propolis can reduce the side effects of gentamicin and the treatment by propolis as remedial effects is better than protective effects in each of enzyme assays, gene expression of TNF α, NFK B, Nephrin and Caspase 3 and histological studies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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