| Autor: |
Girmatsion, Mogos1,2, Adhanom, Awet2,3, Gebremedhin, Henok2,3, Mahmud, Abdu1,2, Yunfei Xie1, Yuliang Cheng1, Hang Yu1, Weirong Yao1, Yahui Guo1 guoyahui@jiagnan.edu.cn, He Qian1 |
| Předmět: |
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| Zdroj: |
Journal of Environmental Science & Health. Part A. Toxic/Hazardous Substances & Environmental Engineering. 2021, Vol. 56 Issue 8, p886-895. 10p. |
| Abstrakt: |
Hg2+ toxicity is one of the most common chemical poisonings that occurs mainly from drinking polluted water. In the current work, Phycocyanin (PC) was exploited as a fluorescent sensor for sensitive and selective detection of Hg2þ in an aqueous system. PC-Hg2þ interaction was monitored using a spectro-fluorometer under different buffered solutions at pH values of 6,7,8,9, or 10 above the isoelectric point of PC (5.18). A remarkable decrease of PC fluorescence intensity was observed under Tris-buffer at pH 6 upon the addition of increasing Hg2+ concentrations (1-120 nM). Under the maintained experimental conditions, the current sensor showed a good linear relationship with R² = 0.9971 and a limit of detection as low as 0.7 nM was achieved. In addition, a notable selectivity of Hg2þ over other nine heavy metals (Cu2+, Zn2+, Pb2+, Mg2+, Mn4+, Li+,F e3+, Co2þ,andAl3+) was achieved in the presence of 120 nM of each metal. Moreover, the current fluorescent detection assay was also tested in real samples of pond water, and recoveries as well as relative standard deviations within the acceptable limits were recorded. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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