| Autor: |
Zhou, Guiqing1,2 (AUTHOR), Liu, Jianhui1,2 (AUTHOR), Li, Xiangyang1,2 (AUTHOR), Sang, Yujian1,2 (AUTHOR), Zhang, Yue1,2 (AUTHOR), Gao, Leqiang1,2 (AUTHOR), Wang, Ji1,2 (AUTHOR), Yu, Yang1,2 (AUTHOR), Ge, Wei3 (AUTHOR), Sun, Zhiwei1,2 (AUTHOR), Zhou, Xianqing1,2 (AUTHOR) xqzhou2@163.com |
| Předmět: |
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| Zdroj: |
Environmental Pollution. May2021, Vol. 277, pN.PAG-N.PAG. 1p. |
| Abstrakt: |
Silica nanoparticles (SiNPs) could cause reproductive toxicity. The role of miRNAs in reproductive toxicity induced by SiNPs is still ambiguous. The present study was designed to investigate the role of miRNA-450 b-3p. In vivo , 40 male mice were randomly divided into control, and 20 mg/kg SiNPs groups. The mice were administrated by tracheal perfusion for 35 days. In vitro , spermatocyte cells (GC-2spd cells) were divided into 6 groups: 0 μg/mL SiNPs groups, 5 μg/mL SiNPs groups, 5 μg/mL SiNPs + miRNA-450 b-3p mimic transfection group, 5 μg/mL SiNPs + miRNA-450 b-3p mimic negative control group, 5 μg/mL SiNPs + miRNA-450 b-3p inhibitor transfection group, and 5 μg/mL SiNPs + miRNA-450 b-3p inhibitor negative control group. The results showed that SiNPs induced the apoptosis of spermatogenic cells, decreased the quantity and quality of the sperm, reduced the expressions of miR-450 b-3p, and increased the protein expressions of the MTCH2, BID, BAX, Cytochrome C, Caspase-9, and Caspase-3 in the testis. In vitro, the mimic of miRNA-450 b-3p reversed the decrease of viability and the increase of apoptosis rate and significantly antagonized the expression enhancements of the MTCH2, BID, BAX, Cytochrome C, Caspase-9, Caspase-3 induced by SiNPs, while inhibitor of miRNA-450 b-3p further promoted the effects induced by SiNPs. The result suggested that SiNPs could inhibit the miR-450 b-3p expression resulting in activation of the mitochondrial apoptosis signaling pathways by regulating the MTCH2 in the spermatocyte cells and, thus, induce the reproductive toxicity. [Display omitted] • miRNA-450 b-3p could target and regulate the expression of MTCH2. • SiNPs inhibited miRNA-450 b-3p expression resulting in targeting promotion of MTCH2 expression. • SiNPs activated spermatocyte apoptosis via MTCH2-mediating mitochondrial signaling pathway. • miRNA-450 b-3p might play important role in apoptosis of spermatocyte cell via MTCH2. Main Finding: SiNPs induce the spermatocyte cell apoptosis by inhibiting the expression of the miR-450 b-3p form target mitochondrial apoptosis signaling pathway in vivo and vitro. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
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Full Text from ScienceDirect