| Abstrakt: |
During its development, embryo is easily susceptible to reactive oxygen species (ROS). Evidence demonstrate protective role of the antioxidants, improving both cellular growth and embryonic development. Among these, ellagic acid (EA) is a natural antioxidant with anti-inflammatory and anti-carcinogen properties. The aim of this work was to assess in vitro the protective and anti-genotoxic role of EA during Danio rerio (zebrafish) embryonic development. For the study, zebrafish embryos were treated with H 2 O 2 (15 μM, 30 μM and 45 μM) to simulate an oxidative damage, and with EA (2.5 mM, 5 mM and 10 mM) for 8, 20, 24, 48, 96 hpf (hours post fertilization). Vitality rate, alterations in the morphology and behavior of the larvae and the genomic stability were analyzed. The exposure to H 2 O 2 caused genotoxicity for all exposure times. The incubation in 45 μM H 2 O 2 and 30 μM H 2 O 2 resulted in increased mortality rate of the larvae, as well as 10 mM EA. The co-exposure was performed using to 15 μM H 2 O 2 and 2.5 mM and 5 mM EA and it demonstrated the EA capacity to protect the embryo DNA and development from the oxidative insult. Particularly, the co-exposure to 15 mM H 2 O 2 and 5 mM EA showed an increase in the embryo survival rate and absence of alterations in morphology and behavior at 96 hpf. Interestingly, we observed a higher genomic stability at 8h and 20h co-exposure (15 mM H 2 O 2 and 5 mM EA) time. The decline observed in ROS concentration for both exposure times confirmed the observation. In conclusion, EA protects the zebrafish embryonic development from DNA oxidative damage increasing the embryo survival rate and improving morphological parameters of the larvae. • Low concentrations hydrogen peroxide induces zebrafish embryo abnormal development. • Ellagic acid protect zebrafish embryonic development against hydrogen peroxide. • Ellagic acid counters DNA damage caused by hydrogen peroxide in zebrafish embryo. • Ellagic acid reduces intracellular ROS produced by hydrogen peroxide. • Ellagic acid increases embryo DNA genomic stability reduced by hydrogen peroxide. [ABSTRACT FROM AUTHOR] |
Full Text from ScienceDirect