| Autor: |
Olah, Zita1, Bush, Ashley I.2, Aleksza, David3, Galik, Bence4, Ivitz, Eszter1, Macsai, Lilla1, Janka, Zoltan1, Karman, Zoltan1, Kalman, Janos1, Datki, Zsolt1 datki.zsolt@med.u-szeged.hu |
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| Zdroj: |
Ecotoxicology & Environmental Safety. Oct2017, Vol. 144, p115-122. 8p. |
| Abstrakt: |
Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18 S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (H 2 O 2 ) and sodium azide (NaN 3 ) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC) , indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo . This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
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