| Autor: |
Seemann, Frauke1, Jeong, Chang-Bum2, Zhang, Ge3, Wan, Miles Teng1, Guo, Baosheng3, Peterson, Drew Ryan1, Lee, Jae-Seong2, Au, Doris Wai-Ting1 bhdwtau@cityu.edu.hk |
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| Zdroj: |
Aquatic Toxicology. Feb2017, Vol. 183, p127-134. 8p. |
| Abstrakt: |
Benzo[a]pyrene (BaP) at an environmentally relevant concentration (1 μg/L) has previously been shown to affect bone development in a transgenerational manner in F3 medaka ( Oryzias latipes ) larvae (17 dph). Here, we provide novel histomorphometric data demonstrating that the impaired bone formation at an early life stage is not recoverable and can result in a persistent transgenerational impairment of bone metabolism in F3 adult fish. A decrease in bone thickness and the occurrence of microcracks in ancestrally BaP-treated adult male fish (F3) were revealed by MicroCt measurement and histopathological analysis. The expression of twenty conserved bone miRNAs were screened in medaka and their relative expression (in the F3 ancestral BaP treatment vs the F3 control fish) were determined by quantitative real-time PCR. Attempt was made to link bone miRNA expression with the potential target bone mRNA expression in medaka. Five functional pairs of mRNA/miRNA were identified (Osx/miR-214, Col2a1b/miR-29b, Runx2/miR-204, Sox9b/miR-199a-3p, APC/miR-27b). Unique knowledge of bone-related miRNA expression in medaka in response to ancestral BaP-exposure in the F3 generation is presented. From the ecological risk assessment perspective, BaP needs to be regarded as a transgenerational skeletal toxicant which exerts a far-reaching impact on fish survival and fitness. Given that the underlying mechanisms of cartilage/bone formation are conserved between medaka and mammals, the results may also shed light on the potential transgenerational effect of BaP on skeletal disorders in mammals/humans. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
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Full Text from ScienceDirect