| Abstrakt: |
The doubly labeled water (DLW) method is commonly used to measure energy expenditure in free-living wildlife and humans. However, DLW studies involving animals typically require three blood samples, which can affect behavior and wellbeing. Moreover, measurement of H (δ2H) and O (δl8O) isotope concentrations in H2O derived from blood using conventional isotope ratio mass spectrometry is technically demanding, timeconsuming, and often expensive. A novel technique that would avoid these constraints is the real-time measurement of δ2H and δ18O in the H20 vapor of exhaled breath using cavity ring-down (CRD) spectrometry, provided that S2H and δ180 from body H20 and breath were well correlated. Here, we conducted a validation study with CRD spectrometry involving five zebra finches (Taeniopygia guttata), five brown-headed cowbirds (Molothrus ater), and five European starlings (Sturnus vulgaris), where we compared δ2H, δ18O, and rCO 2 (rate of CO2 production) estimates from breath with those from blood. Isotope concentrations from blood were validated by comparing dilution-space estimates with measurements of total body water (TBW) obtained from quantitative magnetic resonance. Isotope dilutionspace estimates from δ2H and δ18O values in the blood were similar to and strongly correlated with TBW measurements (R2 = 0.99). The 2H and18O (ppm) in breath and blood were also highly correlated (R2 = 0.99 and 0.98, respectively); however, isotope concentrations in breath were always less enriched than those in blood and slightly higher than expected, given assumed fractionation values between blood and breath. Overall, rCO 2 measurements from breath were strongly correlated with those from the blood (R2 = 0.90). We suggest that this technique will find wide application in studies of animal and human energetics in the field and laboratory. We also provide suggestions for ways this technique could be further improved. [ABSTRACT FROM AUTHOR] |
