Reduced zinc cytotoxicity following differentiation of neural stem/progenitor cells into neurons and glial cells is associated with upregulation of metallothioneins.

Autor:	Nishikawa, Mayu1, Mori, Hideki1, Hara, Masayuki1 hara@b.s.osakafu-u.ac.jp
Předmět:	*Cell-mediated cytotoxicity Metallothionein Zinc toxicology Neural stem cells Neuroglia Gene expression Cell differentiation Progenitor cells
Zdroj:	Environmental Toxicology & Pharmacology. May2015, Vol. 39 Issue 3, p1170-1176. 7p.
Abstrakt:	We investigated zinc cytotoxicity in mouse neural stem/progenitor cells (NSPCs) and their differentiated progeny (neuronal/glial cells) in correlation with expression of metallothionein (MT) gene. Differentiated cells were less sensitive than NSPCs to ZnCl 2 (IC 50 : 128 μM vs. 76 μM). Differentiation of immature NSPCs to the differentiated cells led to an increase in expression of MT family genes ( Mt1 , Mt2 , Mt3 , and Mt4 ). Zinc exposure induced a dose-dependent increase in expression level of Mt1 and that of Mt2 in both NSPCs and the differentiated cells. Our results showed that the reduced cytotoxicity of zinc associated with differentiation from NSPCs into their progeny was related to the upregulation of MTs. [ABSTRACT FROM AUTHOR]
Databáze:	GreenFILE
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