Vliv dlouhodobého podávání morfinu na opioidní receptory v mozkové kůře potkana
| Autor: | Jeřábková, Kateřina |
|---|---|
| Jazyk: | čeština |
| Rok vydání: | 2012 |
| Předmět: |
[3H]diprenorphine - central nervous system - DAMGO - G-protein - competitive binding experiments - µ-opioid receptor - morphine - saturation binding experiments - tolerance - equilibrium dissociation and inhibition constant
[3H]diprenorphine - centrální nervová soustava - DAMGO - G-protein - kompetiční vazebné pokusy - µ-opioidní receptor - morfin - saturační vazebné pokusy - tolerance - rovnovážná disociační a inhibiční konstanta |
| Druh dokumentu: | masterThesis |
| Popis: | 5- ABSTRACT A huge effort has been put in determining the mechanism of the development of tolerance and dependence in context of clinical use of morphine for treatment of severe pain. Understanding of this mechanism would help to design new and more efficient pharmaceuticals. This diploma paper discus the opiate receptors with a special focus on long-term effect of chronic morphine treatment, which was determined using a radioligand binding assays with a non-selective antagonist [3 H]Diprenorphine. One of the goals of this work was to create and optimise a method for preparation of pure plasma membranes from rat cortex using percoll gradient. There were five groups, which differed in the length of morphine treatment: ten days (M-10), twenty-eight days (M-28), ten days with seven days of regression (RM-10 twenty-eight days with seven days of regression (RM-28) and a control group (K). The loss of total opioid receptor number was noticeable after ten days and grew slightly during continuous morphine treatment and kept lowering in the period of regression. The total loss was approximately 30% of the control binding. The equilibrium dissociation constant (Kd), thus the affinity of [3 H]Diprenorphine wasn't significantly different among the groups. Morphine acts through µ-opioid receptor, that's why there was a... |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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