| Popis: |
This work brings extending of applications of sequential injection analysis in area of pharmaceutical analysis. It is focused on integration of monolithic columns into SIA system that enables simultaneous determination of few analytes in samples with matrix (biologic samples) or simple mixtures (pharmaceutical preparations). A new created method was called Sequential Injection Chromatography (SIC) and represents a new generation of flow methods. Integration of short monolithic column with low back-pressure leads into creation of low pressure flow separation method. Commercially produced monolithic columns with reverse phase sorbent C18 are suitable for determination of active compounds and adjuvants in pharmaceutical preparations. This enables fast determination of samples without or only with simple off-line pre-treatment of sample. Employment of various lengths of monolithic columns used in SIC was studied. First experimental work (appendix I) was first SIC experiment. The aim of it was trouble-free connection of monolithic column with SIA manifold and application on determination of pharmaceutical preparation Diclofenac HBF emulgel (ointment contains diclofenac nitrate, methylparaben and propylparaben). All parts of system were optimized for higher work pressure. Sample of ointment was... |
