Transferring Method with Photocurable Resin for Preparation of Surface-enhanced Raman Scattering Substrates
| Autor: | Hung-Yi Huang, 黃弘毅 |
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| Rok vydání: | 2019 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 In this study, a new method was developed to prepare active substrates for surface-enhanced Raman scattering (SERS) measurements. In this method, metal nanostructures are first prepared on Ge crystal by electroless displacement method. Following addition of photocurable resin on the metal nanostructure and cured with UV light, metal nanostructures can be transferred from Ge surface to the film of the cured resin. By this way, the metal nanostructures are partially buried in the photocurable resin to significantly increase the stability of the metal nanostructure. Also, Ge crystal is used only for production of metal nanostructure, which can reduce the cost in production of SERS substrates. Meanwhile, this method can be applied to form array sensing chip with just few additional steps. In order to understand the influence of various factors in fabrication of SERS substrates in this proposed method, the basic properties of photocurable resins, the factors affecting transferring efficiencies, and the metal nanostructures on different solid supports were examined in sequence. Because our goal is to prepare high performance SERS substrates, prepared substrates were also scanned their SERS spectra to correlate the examined factors. Results indicated that organic solvent was necessary to dilute the photocurable resin for controlling the film thickness of the cured photocurable resin and the efficiency in transferring the metal nanostructure. To polymerize the photocurable resin, an irradiation time longer than 4 min was generally required. Metal nanostructures of Ag, Au, Pt, and Cu on Ge crystal and different solid supports were examined. Results indicated that Ag nanostructures provided the best performance among these examined metals. With the optimal conditions, the prepared SERS substrates were also applied to detect analogs of nucleobases. The spectral features of these compounds could be clearly observed from the SERS spectra detected by the SERS substrates prepared in this work. Based on the detection of adenine, prepared SERS substrates exhibit an EF of 3.9107 and the linear range was up to 50 µM with a detection limit of 0.4 µM. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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