Combination of chemical assay and bioassay to determine brominated contamination in dust and their toxicity in the elementary schools, kindergartens and automobiles
| Autor: | Gou, Yan-You, 郭彥祐 |
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| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 Indoor dust is an important source of exposure to polybrominated dibenzo-p-dioxins/furans and diphenyl ethers (PBDD/Fs/DEs) for school-age children. Our goal is to investigate concentrations of PBDD/Fs/DEs in elementary school, kindergarten and vehicle dust, and then to use a reporter gene assay (Ad-DR bioassay) to determine concentrations of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in elementary and kindergarten schools and to further assess the impact on school-age children via ingestion. Seventy-eight dusts including 24 normal (NR) and 24 computer classrooms (CR) in elementary school, 10 kindergarten classroom (KR) and 20 vehicle (V) were collected from Southern and Center Taiwan. Fourteen PBDEs and twelve PBDD/Fs were measured using high resolution gas chromatography/high resolution mass spectrometry, whereas total bio-analytical equivalent (BEQ) of PCDD/Fs was measured using Ad-DR bioassay. The mean levels of Σ14PBDEs in NR, CR, KR and V dust were 440, 2331, 453 and 1984 ng/g. The means of ΣPBDD/Fs and ΣPCDD/Fs were 0.0275 ng-WHO2005-TEQ/g (concentration: 4.09 ng/g) and 0.0131 ng-BEQ/g in NR dust, 0.0588 ng-WHO2005-TEQ/g (10.4 ng/g) and 0.0138 ng-BEQ/g in CR dust, 0.0161 ng-WHO2005TEQ/g (2.51 ng/g) and 0.00337 ng-BEQ/g in KR dust, and 0.0790 ng-WHO2005TEQ/g (22.8 ng/g) in V dust. The PBDEs pattern in V dust was quite different from that in other dust samples, but the PBDD/Fs patterns in four types of dust were similar. A linearly significant correlation coefficient (n=78, adjust R2=0.256, p |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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