Detection of Triazole antifungal agents by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Dispersive Liquid-Liquid Microextraction
| Autor: | Sheng-Yu Lin, 林聖育 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 A novel method for the detection of triazole antifungal agents using dispersive liquid-liquid microextraction (DLLME) coupled to surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. Acetone and chloroform were used as the disperser solvent and extraction solvents, respectively. After the extraction, triazole antifungal agents was detected using SALDI/MS with colloidal gold and α-CHCA as the co-matrix. Under optimal extraction and detection conditions, the calibration curve, which ranged from 1.0 to 100.0 nM for posaconazole and from 2.0 to 200.0 nM for itraconazole, was observed to be linear. The limit of detection (LOD) at a signal-to-noise ratio of 3 was 0.3 nM for posaconazole and 0.6 nM for itraconazole. With a sample-to-extract volume ratio of 200, the enrichment factor for triazole antifungal agents was calculated to be 111 and 121 for posaconazole and itraconazole, respectively. This method was successfully applied to the determination of triazole antifungal agents in human urine samples. The LOD was 0.4 nM for posaconazole and 0.9 nM for itraconazole. The recoveries were calculated to the 55.3% and 60.3% for posaconazole and itraconazole, respectively. This method is rapid, eco-friendly, simple, and economic. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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