The genetic analysis with diallel cross for bitter gourd (Momordica charantia L.)

Autor: Sheng-Chih Chang, 張勝智
Rok vydání: 2014
Druh dokumentu: 學位論文 ; thesis
Popis: 102
Bitter gourd is one of the Taiwan''s major vegetable in summer, but it’s rarely studied on genetic characteristics. In order to realize the bitter gourd of agronomic traits, we used 55 inbred lines which were purified in TPSIPS as experience materials. Assay the inbred lines characteristics had significant for fruit length, fruit width, fruit circumference, fruit weight, fruit shape index, flesh thickness, flesh and marrow thickness. They showed diversity and variation with each inbred lines. In order to explore the fruit characteristics correlation co-efficiency, this results showed positively correlation for fruit length, fruit width, flesh thickness and flesh and pith thickness which can use reference. Further the Griffing (1956) and Hayman (1954) diallel cross theory, we explored the combination, heterosis and genetic components. Divided the bitter gourds by fruit shape index, selecting six inbred lines which were 343-2, 48-1, 298, 156-1, 350 and 42-2. We established the 36 crossing combinations (F1). General combining ability effects, only the dry weight is not significant. That means that the other characteristics are significant except additive gene action. The other characteristics are significant. In the early yield components, the first female flowering day and node had the least GCA with 298 inbred line which described the earliness characteristic. However, we can selection the yield traits according to female characters by node numbers at first female flowering and female flower number, but the fruit traits are also very important. The early yield and fruit traits showed that were not focused on the specific inbred line. Specific combining ability effect, at first female flowering nodes and days had the best with 343-2x350 and 343-2x156-1. Female flower number on the main stem from fifteenth to twenty-fifth had the highest with 298x350. On the fruit traits, the fruit length and weight had higher SCA with 298x42-2 and 156-1x48-1. We observed the better performance to be used breeding with those combination. On the gene effects of traits and genetic components, the plant traits were controlled by additive and dominant effects, and additive effects were larger than dominant effects for internode number and internodal length. The positive and negative dominance gene of traits expressed the asymmetric distribution, and controlling the dominance gene were 3-4 groups. Almost all plant traits were controlled by incremental gene. On the flower traits, all characteristics of additive effects were larger than dominant effect. Days to first male flower and ovary length do not have dominant effect, and the other flower traits were partial-dominance effect. On the fruit characteristics, the fruit length, fruit weight and fruit shape index had positive and negative dominance gene of traits express the asymmetric distribution. The addictive effects of all fruit traits were larger than dominant effect. Fruit length, fruit weight and fruit shape index were controlled by 4-5 and 3-4 groups dominance genes. All fruit traits were controlled by partial- dominance and incremental gene effect.
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