Effect of hydroxyapatite contents and the concentration of EDC on the microstructural and biocompatibility of porous collagen/hydroxyapatite composite scaffold

Autor: I-Ching Chen, 陳怡菁
Rok vydání: 2014
Druh dokumentu: 學位論文 ; thesis
Popis: 102
Hydroxyapatite (Ca10(PO4)6(OH)2) and collagen both have great potential choice as biomaterial for bone in orthopedic applications owing to their excellent biocompatibility, bioactivity, osteoconductive properties, and noninflammation and low immunization. The fast bio-degradative rate of collagen in vitro and in vivo test was defect and resulted in limiting the use as biomaterial in the future. Collagen porous scaffold can be treated by cross-linking treatment with glutaraldehyde (GA) and which was an effective way to improve mechanical property and slow down biodegradation rate. However, glutaraldehyde poses with marked cytotoxicity and also can induce mineralization or stiffness in animal tissue. 1-Ethyl-3-(3-dimethyl-aminopropyl-1-carbodiimide) (EDC) generally recognize as a suitable cross linking reagent for collagen based biomaterials because of its mild reaction in cross-linking processing. Therefore, in this study several Col/HAp composite scaffolds were prepared with 1.2 % collagen to different ratios of hydroxyapatite (collagen : hydroxyapatite, 1 : 2.6 (HAp2.6), 1 : 3.0 (Hap3.0), 1 : 3.5 (Hap3.5) and 1：4.0 (HAp4.0), W/W) and cross-linked by different concentrations of EDC (50, 75 and 100 mM). The physicochemical characteristics and biocompatibility of porous collagen/hydroxyapatite composite scaffolds were evaluated to seek an optimal condition for bone biomaterial. The results showed that all Col/HAp composites scaffolds demonstrated spongy, porous structure and the average diameters of pores were ranged from 45~91 μm. The composite scaffolds of HAp 2.6, HAp 3.0 and HAp 3.5 were treated by 75 and 100 mM EDC showed larger average diameter, but there were no significantly differences among all treatments. In the weight loss of scaffolds, lighter weight loss were found in scaffolds of HAp 2.6, 3.0 and 3.5 after cross-linking treatment. In dissolution analysis, scaffolds with cross-linking exhibited a significantly higher retention of mass than that of control groups (P
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