Studies on Outer Membrane Capsular Polysaccharide Transport Protein (wza) Affecting Biofilm Formation in Vibrio vulnificus
| Autor: | Tseng, Kai-Chun, 曾凱群 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Vibrio vulnificus are Gram-negative opportunistic pathogens and form biofilm widely in marine environment. Infection occurs via wound contamination or ingestion of contaminated shellfish. Extracellular polysaccharides of bacteria play critical roles in environment, includes maintaining the structural integrity of the cell envelope, facilitating interactions within bacterial communities, and biofilm formation. The capsular polysaccharide is one of extracellular polysaccharides in Vibrio vulnificus. However, the effect of outer membrane capsular polysaccharide transport protein (wza) on biofilm synthesis remains unknown. This study investigated the effects of outer membrane capsular polysaccharides transport protein (wza) on bacterial growth, bacterial aggregation, biofilm formation, and mRNA expression of extracellular polysaccharide relevant genes including VVA0126, VVA0388, VVA0393, VVA0395, VV1630, VV2041, and VV2043 in Vibrio vulnificus. The results were showed as following: First, the colony of mutant strain JF045 was translucent. Wild type YJ016 and complementary strain JF049 appeared to be opaque. Second, the bacterial aggregations were existed in mutant strain JF045 but absent in wild type YJ016. This manners were shown both in the seawater and freshwater. Third, the formation of biofilm (A490/600) in the mutant JF045 was higher than those in wild type YJ016 and complementary strain JF049 (p |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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