Development of Raman Spectroscopy Flow Cytometry with Combination of SERS Active Nanoprobes for Highly Sensitive Online Multiplex Biosensing
| Autor: | Ling-Hsuan Liu, 劉凌璇 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Pathogen detection is the foundation of disease treatment. The current methods of pathogen detection, for instance, Enzyme-linked immunosorbent assay (ELISA) and Polymerse chain reaction (PCR), require lengthy procedures of cell culture, proliferation and detection. As a consequence, these methods tend to be tedious and time-consuming. Therefore, the development of an accurate and rapid method for analyzing pathogenic bacteria is highly desirable. In this work, we have developed a rapid, high-throughput, and specific bio-sensing platform for detecting particles or bacteria flowing one by one in a Poly(dimethylsiloxane) (PDMS) microfluidic chip using confocal Raman microscopy. We have demonstrated different Raman-labeled Nanoaggregate-embedded beads (NAEBs), each with a Raman reporter such as MGITC, TRITC, XRITC, DTDCI or CV. Furthermore, polystyrene (PS) microspheres of different sizes were used to mimic different bacteria and bio-functionalized for attachment of NAEBs. Results show that the flow cytometry-based system can identify the Surface-enhanced Raman scattering (SERS) signals of PS microspheres and bacteria when attached with Raman-labeled NAEBs. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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