Morphology Conformity and Buffer Enhancement of in vitro Effects on Tissue Culture Cells in Bio Resonance Electric Fields
| Autor: | Li, Cha-Lin, 李佳霖 |
|---|---|
| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Biological Resonance, biological systems will produce an electric field also have zwitter-ion including cell membranes, etc. Both sides of the net charge of the potential difference caused by the difference. In vivo the conductors are discrete conductors by ProGen II 4050 must be given to the polarization of the electric field generated after the charge potential difference. After the action potential starts to open a channel between cells. Cells can reach the cell membrane by channel changes the charge generated and produce a continuous conductor caused by the body's potential and charge conduction. The first step, we used buffer system to do research, four buffer solutions using a buffer closer to the in vivo observation,the buffer is: HBSS,PBS,NaCl, H2O. Give proper energy observed after adding hydrogen peroxide (H2O2) to lerance, Oxidation-Reduction Potential and conductivity produce a change. Focused on the more obvious changes in the number of quality primary research goal HBSS. The second step, observation of cell types can not be given whether the energy cell morphology changes and improved energy metabolism, we use 2Hz/0.24W DC70, 2KHz/0.24W DC50, 2Hz/0.24W DC50, 2KHz/0.24WDC70 four kinds of energy to determine, and 2Hz/0.24W DC70 frequency given frequency can improve the cell type most. The third step, use Seahorse Extracellular Flux Analyzer XF-24 to give the cell energy generated after observing cells changed. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|