The effect of Quercetin in hepatocellular carcinoma
| Autor: | Jie-Fu Chen, 陳界甫 |
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| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 Hetapocellular carcinoma is one of the common cancers in the world, it is also the main reason that makes people die in the cancer. To increase the survival rate or reduce the recurrence rate, it is necessary and important to find those efficacious anti-cancer drugs. Quercetin is common existing in plant with great effect of anti-carcinogenesis and anti- proliferation. It also has good capability on anti-oxidant. Besides, Quercetin has been noticed by the abilities on effecting cell signal transduction and regulating of cell cycle and inducing cell apoptosis. The purpose of this research is trying to observe whether Quercetin can inhibit HCC cell growth by several experiments. Moreover, to find that the mechanism of inhibiting the proliferation、growth、cell cycle、migration and inducing apoptosis on HCC cell by Quercetin, we used microarray so that we can analyze the suppression effect on HCC cell by gene expression. This experiment uses HCC cell line- Hep3 and Huh7 as the materials in vitro culture, and different concentrations of Quercetin are using on experiments of Proliferation, Colony formation, Wound-healing and Flow cytometry. Huh7 is used on microarray analysis due to the better influence on Quercetin as well. Experiment results show that Hep3B and Huh7 have significantly inhibiting proliferation when the concentration of Quercetin is 500, 50μM on 48 hours; it also shows inhibition on colony formation while the concentration is 50、10μM. And Quercetin has good inhibition effect on cell migration after 12 hours when the concentration is 100μM, and it can also induce Hep3B、Huh7 apoptosis amd arrest G1 phase in cell cycle when the concentration is 50、10μM. All effect by Quercetin during the experiments is depending on the concentrations of Quercetin. Moreover, the experiment of microarray analysis for Huh7 also shows that Quercetin can regulate the gene relative to Apoptosis、cell cycle and migration。And these gene regulated by Quercetin may be involve Apoptosis pathway and several signal transduction pathway：JAK/STAT、PI3K/AKT、MAPK/ERK pathway. All the effect on signal transduction pathway or gene regulated by Quercetin are the strong evidence that Quercetin can inhibit the growing of cancer cells、arrest cell cycle、suppress cell migration and induce the apoptosis of cancer cells. Perhaps people can use Quercetin on clinical medicine for curing liver cancer, increase the survival rate and also decrease the side effect and recurrence rate. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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