The difference stiffness of matrix cause MG63 osteoblast-like cell exhibit different behavior when grown on electrospun collagen matrix versus electrospun gelatin matrix
| Autor: | Hau-min Liou, 劉灝泯 |
|---|---|
| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Electrospinning technique is a convenient and efficient method of fabricating nanofiber extracellular matrix (ECM). However, using the fluorinated alcohol solvent in electrospinning fabrication process will cause the protein denaturation and losing the specific charactics. From transmission electron microscopy (TEM) and circular dichroism analysis indicated the collagen dissolving in the hexafluoroisopropanol(HFIP) will lose their original triple helix structure and becoming the random coil structure similar to the gelatin. However, the Young's modulus of the collagen and gelatin matrices were 94.29 ± 15.18 and 71.88 ± 21.10 MPa, respectively, and their ultimate tensile stresses were 1.93 ± 0.37 and 0.93 ± 0.46 MPa,respectively. In this study, we will examine the adhesion, proliferation, osteogenesis-related gene expression and related protein secretion in MG63 osteoblast-like cells that were cultured on the two matrices. From the cell adhesion and proliferation assay we found that there were no significantly differences on these two matrices. However, the gene expression levels of OPN, type I collagen, ALP, and OCN were significantly higher in MG63 osteoblast-like cells grown on the electrospun collagen matrix than in those grown on the electrospun gelatin matrix. In addition, the western blot assay also finding the protein expression of Y397-FAK, S910-FAK, ERK1/2, BSP, and OPN proteins were also higher expression level on the electrospun collagen matrix than on the electrospun gelatin matrix. Above all of date we examine, we speculate that the mechanical properties of the electrospun collagen matrix may induce the osteogenic differentiation ability than electrospun gelatin matrix. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|