Detection of mouse mammary tumor virus (MMTV) -like sequences in human and mice in Taiwan.

Autor: Szu-Peng Wang, 王思芃
Rok vydání: 2010
Druh dokumentu: 學位論文 ; thesis
Popis: 98
Mouse mammary tumor virus (MMTV), an oncogenic B retrovirus, is strongly associated with the development of mouse mammary tumors. Mice might acquire MMTV through endogenous and exogenous routes. LTRs (long terminal repeats) sequences that flank at both ends of virus genome, help virus insert into the DNA of the host and act as enhancers; insertion of an MMTV provirus in the vicinity of a proto-oncogene leads to an inappropriate transcriptional activation of adjacent genes. Several lines of evidences implicate MMTV might be involved in human breast cancer (HBC). The highest incidence of HBC worldwide occurs in lands where Mus domesticus is the native resident or introduced species of house mouse, indicating that M. domesticus might transmit MMTV to human. Recently, companion animals, dogs and cats, with frequent human contacts were proposed to contribute to the transmission of MMTV between mouse and man. Our previous study has demonstrated for the first time the presence of MMTV-like sequences in canine and feline mammary gland tumours. Since the prevalence of MMTV like sequences in human and mice in Taiwan is unknown, the goal of present study was set to detect the MMTV-like sequence and transcript in these populations. Specific MMTV-like env sequences were found in 5.8 % and 8.5 % of mice blood and human breast cancer, respectively. Moreover MMTV env RNA was present in 5% (1/19) of dogs/cats samples that that were previous detected positive in MMTV sequences, implying that pet animals might play a role in transmission of MMTV like virus. The incidence rate and the phylogentic relation of these sequences enable us to explore the involvement of MMTV in cancer and also the possible role that companion animals play in MMTV transmission.
Databáze: Networked Digital Library of Theses & Dissertations
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