THE STUDY OF MICROFLUIDICS WITH POROUSSEMI-PERMEABLE MEMBRANE STRUCTURE FOROPTICAL FIBER BIOSENSOR BASED ON SURFACE PLASMON RESONANCE
| Autor: | Ren-Kun Liang, 梁仁坤 |
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| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 The phenomenon of surface plasmon resonance (SPR) is widely applied to the biological and chemical sensing for characterizing and quantifying bio- molecular interactions. The traditional advantages of SPR detective method have high sensitive response, kinetic study in real-time, and label-free for biological sensing. Optical fiber sensor has the characteristics of small, low cost, and the same scheme on conventional SPR technique. It is worthy to develop the optical fiber SPR sensor. Recently the flu season is upon us, so Virus detection and preventive issues to be proposed again. So, the real-time detection is a very important research topic. At present, the microfluidics system design is easily for the feasibility in real-time detection. Therefore, development of a small system, low-cost, and disposable easy to use with simple biosensor microfluidics is important, But the biosensor micro-fluidics design is quite complicated and difficult to control the performance. Using porous membrane with a wicking pad absorber to drive the test solutions by capillary phenomena has been carried out as the microfluidics structure in SPR measurement. Besides, a laser diode operated at 690 nm wavelength as the light source was applied to analyze the SPR responses of DI water, ethanol, and glycerin solutions. The results show that in the case of porous semi-permeable membrane structure for the side-polished fiber SPR sensors possess very good repetitive ability and excellent stability in the SPR responses than the original fiber sensor, and can enhanced the sensitivity for the Biotin-BSA sensing. The porous semi-permeable membrane structure we proposed can reduce the reagent dose in sensing and without any residual in the system, and provides the real-time detection ability and convenience in handling in the measurements. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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