The mechanism of abnormal hemorheology in diabetic rat and high fructose-fed rat
| Autor: | Ming-Tsung Chen, 陳銘宗 |
|---|---|
| Rok vydání: | 2008 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 The present study based on hemorheological model was to evaluate changes in hemorheological parameters of diabetes rats and normal rats fed with high fructose solution, and to discuss the possible mechanism response for these changes. The animal study was divided into two sections: the diabetes rats and the high fructose-fed rats using 8-weeks-old male Wistar rats. The rats in the diabetes group was induced by a single intraperitoneal (i. p.) injection of 50 mg/kg streptozotocin, 15 min after the i.p administration of 180 mg/kg of nicotinamide which was used to protect the insulin-secreting beta-cells. The rats of the high fructose fed group were freely supplied with 21% fructose aqueous solution as a result of generating X syndrome. Rats at similar age were adopted as the controls. Blood samples were collected following 14 hours fasting and measured for biochemical parameters, complete blood counts and hemorheological parameters. The results revealed that there were significant differences in all hemorheological parameters of the diabetes and the high fructose-fed groups from the control group. Whole blood viscosity, erythrocyte aggregation, fibrinogen levels, and free radicals in the diabetes and the high fructose-fed groups were significantly higher than that of the control group, while erythrocyte deformability and volume flow rate of MC-FAN of the diabetes and high fructose-fed groups were lower than that of the control group. The low volume flow rate measured in the diabetes and high fructose-fed groups was probably due to the rise of whole blood viscosity that was caused by high erythrocyte aggregation at low shear rate and low erythrocyte deformability at high shear rate. Therefore, we suggest that abnormal hemorheological properties generated by two animal models we employed in the present study could be developed as a useful animal hemorheological plateform for selection of drugs. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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