The Study of Swine Salmonella Antibody by Oral Administration

Autor: YU-MING CHI, 紀又銘
Rok vydání: 2007
Druh dokumentu: 學位論文 ; thesis
Popis: 95
The content of abstract in this thesis: Due to the abusage of antibiotics in the field for these years, together with the explosion of classical swine fever and EHEC and the respiratory diseases. All these factors make Salmonella spp. reoccur constantly and periodically, and make the disease widespread speedily with a heavy financial loss to the breeders. This make the administrative staff get more difficult to conduct any prevention. This study is focused on the marketing product of Salmonella spp. IgY to conduct its quality, stability and potential activity of specific and the field test. At the tested result shows, the IgY antibody could be assured its certain specific antibody IgY existing through SDS-PAGE and Western blotting assay. This specific antibody IgY could be achieved an extremely stability after process under the environment at pH 2 ~ 11 for two hours and under a temperature of 100℃ for 5 minutes. And this specific antibody IgY shows a significant agglutinated response with Salmonella choleraesuis and Salmonella typhimurium. Attacked with ten doses of 108 CFU/ml of Salmonella choleraesuis and Salmonella typhimurium, via oral administration to 6-week-old three-interbreed piglets, after making these piglets to suffer from the illness, then treat them with the antibody IgY. And found there is a significant improve on the appearance symptom, mortality rate and contains bacterium quantity in feces. Key words: Swine, IgY, Salmonella
Databáze: Networked Digital Library of Theses & Dissertations