Expressions of hormone receptors, KIT and HER-2 in canine mast cell tumor

Autor: Yu-Lin Kuo, 郭俞麟
Rok vydání: 2007
Druh dokumentu: 學位論文 ; thesis
Popis: 95
Mast cell tumor (MCT) is one of the most common tumors in dogs, accounting for approximately 7 %- 21 % of canine cutaneous and subcutaneous neoplasms. Prognosis of MCT varies from case to case, therefore plenty of predictors were evaluated. Histological grading is considered the most powerful prognostic parameter up to date. Sex predisposition has been studied but remains controversial. The expressions of estrogen receptors (ER) and progesterone receptors (PR) have been widely studied in mammary gland tumors both in human and canine and are confirmed to be good prognosticators. ER and PR were found in canine MCTs according to a literature published in 1982. Over-expressions of HER-2 were found in several kinds of human and canine neoplasms including breast cancer and some soft tissue sarcoma of mesenchymal origins. The aim of this study is to determine the expressions of ER, PR, HER-2 and KIT immunohistochemically and to evaluate the consequencing clinical relevance. Samples were collected from NCHU-VMTH between 1999 and June of 2006. Each sample was examined histopathologically with routine H&E stain and four immunohistochemical methods that detect ER, PR HER-2 and KIT, respectively. 20 cases met the criteria of sample colleting and were enrolled in this study. 4 out of 20 (20 %) samples were PR- positive, but none of the nuclei of all 20 specimens was stained positive of ER. ER-positive staining was found in the cytoplasm of seven samples (35%). This phenomenon implied the expression of non-functioning ER present in cytoplasm of MCT cells. KIT protein was found in 95 % of samples. The IHC staining pattern was related to the histological grading of tumor cells. Because either ER or HER-2 were not expressed in all of the samples in this study, the use of chemotherapy against ER or HER-2 would not be suggested to be applied to MCT patients. The relationship between the expression of PR and pathogenesis of MCT requires further investigation.
Databáze: Networked Digital Library of Theses & Dissertations
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