Study on the virulence factors of Klebsiella pneumoniae
| Autor: | Hung-Ting Liao, 廖虹婷 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Klebsiella pneumoniae is an important pathogen of nosocomial and community-acquired infections, especially suppurative infection, pneumonia, urinary tract infection, and septicemia in humans. Althogh the development of new and potent antibiotics, K. pneumoniae infectious are still associated with a high mortality rate up to 50%. In Taiwan, the high incidence of K. pneumoniae pyogenic liver abscess has never reported in English literature. In addition, extrahepatic metastases, such as septic endophthalmitis, are often occurred with serious complications. The high mortality rate and high incidence of K. pneumoniae pyogenic liver abscess in Taiwan make further investigation is necessary. The current researches, we have known several virulence factors of K. pneumoniae liver abscess : (1) rmpA; (2) rmpA2; (3) magA. In this study, the pvf (pneumoniae virulence factor) mutant was isolated by mini-Tn5 transposon insertional inactivation and a serum sensitivity assay. The pvf mutant was significantly more easily killed by human serum than the wild type. The colony diameter of this mutant on 0.3% soft agar plate was larger than those of wild type strain. Interestingly, the mice experiments demonstrated virulence attenuation when the pvf mutant was administered intraperitoneally to normal mice. In addition, the cytokine expression profile in lung tissue of mice infected by pvf mutant showed significantly different from those of mice infected by wild type, suggesting that the pvf factor may mediate resistance to host innate defenses and it is an important virulence factor for mice. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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