Optimization of the cultivation conditions on the levan production from Bacillus subtilis fermented soybean and black soybean
| Autor: | Chia-Hung Weng, 翁嘉鴻 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 Levan as the polymer on the basis of fructose. It’s part of fibre which help probiotic growth in the intestines and human health. This study investigated optimization of the cultivation conditions on the levan production from Bacillus subtilis fermented soybean and black soybean. This experiment uses is such four strains as Bacillus subtilis BCRC 14714, Bacillus subtilis BCRC 14715, Bacillus subtilis BCRC 14716, Bacillus subtilis BCRC 14718, carry on the fermented test on the black soybean (Tainan No.3), black soybean (Tainan No.5) and black soybean (Kaohsjung No.7), compare production capacity of levan. The result shows Bacillus subtilis BCRC 14715 production of levan more than other strains 1-1.5 fold. On the levan production from soybean and black soybean compare. The result shows black soybean (Kaohsjung No.7) production of levan more than black soybean (Tainan No.3) and black soybean (Tainan No.5) 2 fold. In this experiment black soybean (Kaohsjung No.7), soybean and Bacillus subtilis BCRC 14715 are our use materials and starin. It’s investigating different inoculum (102~108 CFU/g)、different fermented time ( 4~24 hours) and different fermented temperature (30℃~55℃) effect on the levan production. And use response surface method (RSM) probe into Optimization of the cultivation conditions on the levan production. The result shows the amount of inoculum connected levan reduce relatively high. The inoculum as 102 CFU/g has higher yield. The soybean and black soybean yield are 0.68 % and 0.80 % respectively. Effect of investigating different fermented temperature on the levan production. The result shows soybean at 35℃ had higher yield on the dry base 0.71%, and black soybean at 40℃ had higher yield on the dry base 0.84%. Effect of investigating different fermented time on the leavn production. The result shows soybean and black soybean at the 12 hours had higher yield, on the dry base 0.74 % and 0.83 % respectively. Exercise response surface method analysis the optimization of the cultivation conditions on the levan production. We are receive the optimization on the levan production of soybean sample is : bacteria inoculated at 103 CFU/g, fermentation time for 12 hours, fermentation temperature at 35℃. Levan yield on the dry base 0.84%. The optization on the levan production of black soybean sample is: bacteria inoculated at 103 CFU/g, fermentation time for 12 hours, fermentation temperature at 38℃. Levan yield on the dry base 1.12%. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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