Surface-imprinted polymer particles for the recognition of saccharides
| Autor: | Syuan-De Su, 蘇軒德 |
|---|---|
| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 This research is designed to generate binding sites on the surface of polymer particles for the recognition of saccharides by inverse suspension polymerization. A surfactant-like template molecule,alkyl-galactose and alkyl-maltose, was synthesized in three steps. The hydroxyl groups of saccharide were protected by reacting with acetic anhydride to give peracetylated saccharide. Peracetylated β-saccharide was alkylated by reacting with alkyl alcohol component and then deprotected by triethylamine to give the target template molecule, alkyl-glycosides(octadecyl-β-D-galactopyranoside、dodecyl-β-D-galactopyranoside、decyl-β-D-galactopyranoside、octadecyl-β-D-maltopyranoside、dodecyl- β-D-maltopyranoside). A functional monomer, m-acrylamidophenylboronic acid, was synthesized by the amidation of m-aminophenylboronic acid with acrylic acid. To study the possibility of preparing high-crosslinked polymer particles by inverse suspension polymerization, span®60 was employed as the surfactant for model study. First, the preparation of polymer particles with acrylamide and N,N’-ethylenebisacrylamide by inverse suspension polymerization was investigated thoroughly in terms of reaction times, the mole fraction of monomer to crosslinking agent, stirring speed, concentration of surfactants (Span®60), concentration of initiators (4, 4’-azobis(4-cyanovaleric acid)), the relative amount of methanol to water. The results show that 50 mole% crosslinking-agent polymer particles with the size of 20.1±3.3 μm were prepared successfully at 55℃ in 3 hours under the following operating conditions: the stirring rate is 800 rpm, the surfactant’s concentration is 9.7 mM, the initiator’s concentration is 0.001 grams and the relative amount of methanol to water is 25%. Then acrylamide and span®60 were replaced by m-acrylamidophenylboronic acid and alkyl-glycosides. The results show that 50 mole% crosslinking-agent polymer particles with the size of ~1.5 μm were prepared successfully at 50℃ in 3 hours under the following operating conditions: the stirring rate is 800 rpm, the surfactant’s concentration is 14.6 mM, the initiator’s concentration is 0.001 grams, the relative amount of methanol to water is 40%, the relative amount of dodecyl-β-D-galactopyranoside to span®60 is 30% and using NH4Cl＋NaOH buffer solution with pH 7.4. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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