Cigarette smoke extracts induced DNA damage in human umbilical vein endothelial cells
| Autor: | Yu-Han Chuang, 莊玉涵 |
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| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 Cigarette smokeing is a major cause of human cancer and cardiovascular diseases, although its mechanisms remain unclear. Cigarette smoke can be divided into two phases, gas phase and particulate matter (tar). Both phases contain high concentrations of oxidants and free radicals, especially nitric oxide (NO) and nitrogen oxides in the gas phase and quinine/hydroquinone complex in tar. We found that both filtered and non-filtered cigarette smoke extracts (CSE) had similar NO level, but they did not induce calf thymus DNA strand breaks in vitro. Human umbilical vein endothelial cells were exposed to CSE, and DNA damage detected by alkaline comet assay, showed a dose-dependent manner, however, non-filtered CSE induced more DNA damage than filtered CSE. These data suggest that (1) aqueous CSE induced DNA strand breaks may be through a metabolic activation pathway, (2) NO in aqueous CSE may not be the major cause that is responsible for the generation of DNA strand breaks. We also determined CSE-induced expression of cell adhesion molecules in human umbilical vein endothelial cells. Cells were exposed to CSE, and fluorescence intensity was measured by the flow cytometric assay. It was found that CSE induced the surface expression of intercellular adhesion molecule 1（ICAM-1）in human umbilical vein endothelial cells. Also, we investigated the binding activity of transcription factor activator protein 1（AP-1）by electrophoretic mobility shift assay（EMSA）. Results showed that CSE induced AP-1 binding activity. These results suggested that the expression of ICAM-1 induced by CSE may be related to the binding activity of AP-1. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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