The study on the in-vitro stability of Tc(V)-99m DMS and the assessmeot of bone and joint infections
| Autor: | Lee, Bi-Fang, 李碧芳 |
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| Rok vydání: | 1997 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 85 Tc(V)-99m DMS, developed by Yokoyama et al in 1981, has been recognized to be advantageous for the scintigraphic diagnosis of various malignant tumors and their metastasis. The aim of this study is to assess the in-vitro stabilityof Tc(V)-99 m DMS. Thin-layer chromatography, including paper chromatographya nd silica gel thin layer chromatography, is performed to determine the change of radiochemical species presented in the reconstituted solution of Tc(V)-99mDMS prepared from the DMS kit(Institute of Nuclear Energy Research, Atomic Energy Council) and the commercial DMSA kit(Nephroscint, IRE CELLTARG Radiopharmaceuticals). The bioscan imaging scanner is used to measure the Rf value and labeling efficiency of radiochemical species on the chromatographic strip. Thein-vitro stability of Tc(V)-99m DMS prepared from the DMS kit and the commercialDMSA kit is studied by examining various parameters which include tempera-ture(℃) and time(hr) after reconstitution. The results show decreased radiola-beling efficiency of Tc(V)-99m DMS prepared from the DMS kit and the commercialDMSA kit as the time elapsed and temperature increased. The in-vitro stabilityof Tc(V)-99m DMS prepared from the DMS kit is actually better than that fromthe commercial DMSA kit. The one-step labeling method of DMS kit is much simplerthan the two-step labeling method of DMSA kit. The aim of the other part of this study is to investigate the diagnostic value of Tc(V)-99m dimercaptosuccinic acid(Tc(V)-99m DMS) in combination with a Tc-99m methylene diphosphonate(Tc-99m MDP) bone scan to localize bone and joint infections compared with Ga-67 scan and Tc-99m MDP bone scan. Thirty-six patients referred for investigation of bone and joint infections were studied. In all patients, bone scan was initially obtained. Subsequently, comparative scans with Tc(V)-99m DMS and Ga-67 were carried out within one week interval. Microbiological and pathologic findings were considered to be proof of the presence of bone and joint infections. Anterior and poste-rior whole-body images and spot images of the suspected area of involvement were acquired with gamma camera. The scans were interpreted by two experienced nuclear medicine physicians blinded to all information other than a clinical suspicion of bone and joint infections. Disagreements were resolved by discussion. The sensitivity, spe-cificity and accuracy were 100%, 93%, and 97%, respectively for Tc(V)-99m DMS and 90%, 93%, and 92%, respectively for Ga-67. Tc(V)-99m DMS was more sensitive and more correct than Ga-67 in the assessment of bone and joint infections, but there was no significant statistical difference between both scans. In addition, no sustantative reports have been made about the efficacy of Tc(V)-99m DMS in the setting of bone and joint infections. Moreover, Tc(V)-99m DMS has many advantages, such as low price, good availability(suitable for daily clinical use), good physical characteristics, no need of blood manipulation and easy preparation procedure. Tc( V)-99m DMS should have the potential of becoming a new modality for checking the bone and joint infections. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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