Studies on the Biopulping of White-rot Fungi

Autor: Lang-Dong Lin, 林蘭東
Rok vydání: 1993
Druh dokumentu: 學位論文 ; thesis
Popis: 81
The major results of the studies are described as follow: (1) Traditional methods of growth rate, weight loss evaluation, Klason lignin analysis and on - media chemical discolored re- action had their shortages in screening suitable fungi for bio- bleaching. Combined spot test method and colorimetry method of- fered good and fast screening for the purpose of bio-bleaching. (2) The flow chart of the reagents and reactions in spot test method forbio-bleaching fungi screening with slices of fungal cultured wood-MEA were as below: a.TMPD 0∼5 min Light pinky purple → purple or blue b.Syringaldazine 20∼30 min Light yellowish green → pink c.Guaiacol 20 min Colorless → reddish brown Pyrogallol 10 min Slight yellow → orange brown d.GA 1 hr Light tea brown → deep brown Guaic 5 min Colorless → guaicum blue Phenol (30 min)∼1 h Colorless → Brown or purple e.Guaic + MEA 4 days Yellowish brown→deep blue→decolored Fugus for biobleaching (3) The obove screened fungi gave higher whiteness in colorimetry method. Different woody materials with different specific fungi while Coriolus versicolor (L.:Fr.) Quel. gave the highest white- ness in all three woody species owing to its highly degraded ab- ility on most components of wood. With suitable culture condit- ions and controlled period, this fungus had potential for apply- ing to non-species specific bio- pulping in subtropical Taiwan. (4) A reverse study in this research by spot testing on commerc- ial enzymes which related to lignin modification or degradation gave the results that traditional used Bavendam method was not suitable for screening and the fungal enzyme system in woody ma- terial was multiple - function.
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