miR-146a is a pivotal regulator of neutrophil extracellular trap formation promoting thrombosis

Autor: Ana B. Arroyo, María P. Fernández-Pérez, Alberto del Monte, Sonia Águila, Raúl Méndez, Rebecca Hernández-Antolín, Nuria García-Barber, Ascensión M de los Reyes-García, Paula González-Jiménez, María I. Arcas, Vicente Vicente, Rosario Menéndez, Vicente Andrés, Rocío González-Conejero, Constantino Martínez
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Haematologica, Vol 106, Iss 6 (2020)
Druh dokumentu: article
ISSN: 0390-6078
1592-8721
DOI: 10.3324/haematol.2019.240226
Popis: Neutrophil extracellular traps (NETs) induce a procoagulant response linking inflammation and thrombosis. Low levels of miR-146a, a brake of inflammatory response, are involved in higher risk for cardiovascular events, but the mechanisms explaining how miR-146a exerts its function remain largely undefined. The aim of this study was to explore the impact of miR-146a deficiency in NETosis both, in sterile and non-sterile models in vivo, and to inquire into the underlying mechanism. Two models of inflammation were performed: 1) Ldlr-/- mice transplanted with bone marrow from miR-146a-/- or wild type (WT) were fed high-fat diet, generating an atherosclerosis model; and 2) an acute inflammation model was generated by injecting lipopolysaccharide (LPS) (1 mg/Kg) into miR-146a-/- and WT mice. miR-146a deficiency increased NETosis in both models. Accordingly, miR-146a-/- mice showed significant reduced carotid occlusion time and elevated levels of NETs in thrombi following FeCl3-induced thrombosis. Infusion of DNAse I abolished arterial thrombosis in WT and miR-146a-/- mice. Interestingly, miR-146a deficient mice have aged, hyperreactive and pro-inflammatory neutrophils in circulation that are more prone to form NETs independently of the stimulus. Furthermore, we demonstrated that community acquired pneumonia (CAP) patients with reduced miR-146a levels associated with the T variant of the functional rs2431697, presented an increased risk for cardiovascular events due in part to an increased generation of NETs.
Databáze: Directory of Open Access Journals
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