VALIDATION OF REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION FOR INDIRECT ASSESSMENT OF FMD VIRUS TITRE

Autor:	D. A. Lozovoy, M. I. Doronin, V. A. Starikov, D. V. Mikhalishin, A. A. Shishkova, A. M. Timina, A. B. Borisov
Jazyk:	English<br />Russian
Rok vydání:	2019
Předmět:	foot-and-mouth disease (fmd) virus infectivity titre validation rt-pcrrt amplifcation threshold cycle Veterinary medicine SF600-1100
Zdroj:	Ветеринария сегодня, Vol 0, Iss 1, Pp 43-50 (2019)
Druh dokumentu:	article
ISSN:	2304-196X 2658-6959
DOI:	10.29326/2304-196X-2019-1-28-43-50
Popis:	Procedure for validation of the method for indirect assessment of cultural FMD virus titre (T FMDV) in raw materials used for vaccine production with real time reverse transcription polymerase chain reaction (RT-PCRrt) based on determined amplifcation threshold cycle value (Ct) using linear regression model, TFMDV= –0.2956Ct+ 11.4650, is described in the paper. Testing results for 390 samples of cultural FMD virus were analyzed and basic validation characteristics of the proposed method: specifcity, accuracy, precision, limit of detection and limit of quantifcation, application scope and linearity, were defned. Validation results for the method for indirect assessment of FMD virus titre with RT-PCRrt met the acceptance criteria.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/ffe916d07ec244cda9686a02215eb376 Zobrazit plný text záznamu View record in DOAJ