Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions
| Autor: | Elena Bobeková, Michal Tomšovský, Petr Horáček |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Vol 56, Iss 2, Pp 281-284 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1211-8516 2464-8310 |
| DOI: | 10.11118/actaun200856020281 |
| Popis: | The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil™ DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR–based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA. |
| Databáze: | Directory of Open Access Journals |
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