Long-term hematologic reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at −80°C in a mechanical freezer
| Autor: | Antonio Galmes, Antonio Gutiérrez, Antonia Sampol, Mariana Canaro, Miguel Morey, Julio Iglesias, Nuria Matamoros, María Antonia Duran, Andrés Novo, María Dolores Bea, Pilar Galán, Josep Balansat, Jordi Martínez, Joan Bargay, Joan Besalduch |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Haematologica, Vol 92, Iss 7 (2007) |
| Druh dokumentu: | article |
| ISSN: | 0390-6078 1592-8721 |
| DOI: | 10.3324/haematol.11060 |
| Popis: | We report the long-term evaluation over 12 years of a simplified technique for stem-cell cryopreservation at −80°C without rate-controlled freezing and with 5% (n=251) or 10% (n=47) DMSO as the sole cryoprotectant. Platelet recovery was greater in the 5% DMSO group while long-term hematologic recovery did not differ. Factors influencing a faster hematologic recovery were infusion of more than 2.7×106/kg of CD34+ cells, 10% DMSO cryopreservation and G-CSF. We confirm that the procedure is feasible with a reduction in infusion-related toxicity from 60% using 5% DMSO. Differences in hematologic reconstitution were not clinically significant if a minimum of 1.5×106/kg CD34+-cells were infused. |
| Databáze: | Directory of Open Access Journals |
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