Tuning the extraction methodology targeting protein-enriched fractions from red algae

Autor: Rafaela Nunes, Pedro Ferreira-Santos, Catarina Moreira, José A. Teixeira, Cristina M.R. Rocha
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Future Foods, Vol 9, Iss , Pp 100335- (2024)
Druh dokumentu: article
ISSN: 2666-8335
DOI: 10.1016/j.fufo.2024.100335
Popis: Alternative protein sources are being studied to substitute animal protein production and decrease the environmental issues that are inherent to the production of animal-based foods.This study investigates the potential of red macroalgae (Gracilaria verrucosa, Porphyra dioica, and Palmaria palmata) as an eco-friendly alternative protein source. Using the Osborne fractionation methodology, proteins were extracted under varying parameters (pH and grinding) and techniques (ultrasound and enzymatic extractions). Seaweed proteins exhibited a strong affinity for water and alkaline environments. Optimal extraction yields were obtained with H2O for G. verrucosa (33.24 ± 1.81 g protein/100 g algae protein) and P. palmata (29.56 ± 1.73 gprotein/100 galgae protein), while P. dioica showed highest yields with aqueous 0.1 M NaOH (26.28 ± 5.90 gprotein/100 galgae protein). Molecular weight patterns revealed aggregates (> 200 kDa) in G. verrucosa and P. palmata in the 0.1 M NaOH fraction. H2O extracts displayed peaks (48–84 kDa) linked to phycobiliproteins, showcasing good emulsifying properties. Solubility varied with solvents, with saline and 70 % EtOH dissolving lower molecular weight proteins. Aqueous 0.1 M NaOH extracts demonstrated promising texturizing capacity.Overall, proteins from red macroalgae show potential for food product development, offering an alternative with lower environmental impact compared to conventional animal-based proteins.
Databáze: Directory of Open Access Journals