| Autor: |
Ofir Israeli, Adi Beth-Din, Nir Paran, Dana Stein, Shirley Lazar, Shay Weiss, Elad Milrot, Yafit Atiya-Nasagi, Shmuel Yitzhaki, Orly Laskar, Ofir Schuster |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
International Journal of Infectious Diseases, Vol 99, Iss , Pp 352-354 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1201-9712 |
| DOI: |
10.1016/j.ijid.2020.08.015 |
| Popis: |
The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharyngeal SARS-CoV-2 swabs. The findings showed an advantage for the extraction procedure; however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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