Identification of a High Affinity Binding Site for Lipo-oligosaccharidic NodRm Factors in the Microsomal Fraction of Medicago Cell Suspension Cultures
| Autor: | Andreas Niebel, Jean-Jacques Bono, Raoul Ranjeva, Julie V. Cullimore |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Molecular Plant-Microbe Interactions, Vol 10, Iss 1, Pp 132-134 (1997) |
| Druh dokumentu: | article |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/MPMI.1997.10.1.132 |
| Popis: | Protease-sensitive binding sites for a 35S-labeled ligand corresponding to the major lipo-oligosaccharidic symbiotic signal of Rhizobium meliloti (NodRm factor), have been identified in the microsomal fraction of Medicago varia cell suspension culture extracts. Binding was reversible and saturable and tetra-N-acetyl chitotetraose was a poor competitor of NodRm binding. Scatchard analysis suggests the presence of a high affinity binding site, termed Nod factor binding site two (NFBS2), with a Kd of 1.9 nM, and perhaps a second site with an affinity (Kd of 70 nM) similar to that of a site (NFBS1) previously characterized in Medicago truncatula root extracts. |
| Databáze: | Directory of Open Access Journals |
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